書誌： Journal of Neuroendcrinology , 2014
Shimogawa Y, Maekawa F, Yamanouch K. Journal of Neuroendcrinology, 26(4); 258-266, 2014.
To determine the relationship between the right and left sides of the ventrolateral ventromedial hypothalamic nucleus (vlVMN) in regulating the expression of oestrogen receptor (ER)a, the unilateral vlVMN was lesioned and the number of ERa-immunoreactive cells and the ERa mRNA level in the intact side of the vlVMN and arcuate nucleus (ARC) were measured in ovariectomised rats. Twenty-four hours after lesioning, brain samples were collected for analysis of ERa expression by immunohistochemistry and the real-time reverse transcriptase-polymerase chain reaction. The number of ERa-immunoreactive cells in the intact side of the vlVMN but not the ARC in the unilateral lesioned group was significantly higher than that in the control or shamlesioned group. Expression levels of ERa mRNA in the intact side of the vlVMN but not the ARC in unilateral lesioned rats were significantly higher than those in the sham-lesioned group. Of transcript variants with alternative 50-untranslated regions (0S, 0N, 0, 0T and E1), the ERa 0 transcript level was significantly increased. These results indicate that unilateral damage of vlVMN induces an increase in ERa in the intact side by increasing ERa transcription in a promoter- specific manner. The findings also suggest the existence of new neuroendocrine control system between the right and left sides for the expression of ERa in the vlVMN.
Key words: unilateral lesion, ventromedial hypothalamic nucleus, oestrogen receptor a, compensation